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- Table of Contents
Cd70 antibody (A02853-2) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Cd70 using anti-Cd70 antibody (A02853-2).
Cd70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd70 Antibody (A02853-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cd70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd70 Antibody (A02853-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Cd70 Antibody Picoband®
Cd70 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Cd70 antibody was positively detected by WB in:
rat spleen tissue, rat thymus tissue, rat lung tissue, rat L6 whole cell, rat PC-12 whole cell, mouse B16 whole cell, mouse spleen tissue, mouse thymus tissue, mouse lung tissue, mouse RAW2647 whole cell, mouse ANA-1 whole cell, mouse SP2/0 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cd70 antibody was positively detected by IHC in:
mouse lymphonode tissue, mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Cd70 Antibody Picoband®
View all CD70 Antibodies
View all CD70 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cd70 recombinant protein (Position: C36-Q191).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
21.9 kDa
Background of Cd70
CD70 (Cluster of Differentiation 70) is a protein that in humans is encoded by CD70 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
Cite This Product
Anti-Cd70 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02853-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
80%
Moderate Homology
Rabbit
67%
Low homology
Sheep
65%
Low homology
Dog
65%
Low homology
Cow
64%
Low homology
Human
63%
Low homology
Monkey
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cd70 antibody
Immunohistochemistry (IHC) protocol for Cd70 antibody
Publications
Application:
Species:
Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma
Published: 2025-02-05
Journal: Scientific Reports
Application: WB
Impact Factor: 3.90
Species: Human
PMID: 39910141
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Product Promise
We promise Anti-Cd70 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02853-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026