Anti-CD8 alpha/Cd8a Antibody Picoband™

CD8 antibody

Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband™ catalog # A02236-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: A02236-3
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

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Product Name

Anti-CD8 alpha/Cd8a Antibody Picoband™

View all CD8 Antibodies

SKU/Catalog Number

A02236-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband™ catalog # A02236-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD8 alpha/Cd8a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse CD8 recombinant protein (Position: K39-K245).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02236-3 is reactive to Cd8a in Mouse, Rat

Applications

A02236-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Background of CD8

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)

Uniprot ID

P01731

Gene ID

12525

Gene Name

Cd8a

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

27.456kDa

Alternative Names

53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker Cd8a|BB154331, Ly-, Ly-2, Ly-3, Ly-35, Ly-B, Lyt-, Lyt-2|CD8 antigen, alpha chain|T-cell surface glycoprotein CD8 alpha chain|Lyt-2.1 lymphocyte differentiation antigen (AA at 100)|T-cell surface glycoprotein Lyt-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd8a, check out the Cd8a Infographic

Cd8a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A02236-3 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation

Liu Q,Hao Y,Du R,Hu D,Xie J,Zhang J,Deng G,Liang N,Tian T,Käsmann L,Rades D,Rim CH,Hu P,Zhang J.Radiotherapy programs neutrophils to an antitumor phenotype by inducing mesenchymal-epithelial transition.Transl Lung Cancer Res.2021 Mar;10(3):1424-1443.doi:10.21037/tlcr-21-152.PMID:33889520;PMCID:PMC8044478.
Species: Mouse
A02236-3 usage in article: APP:IHC, SAMPLE:SPLEEN AND LYMPH NODES, DILUTION:1:400

Dai X,Lu L,Deng S,Meng J,Wan C,Huang J,Sun Y,Hu Y,Wu B,Wu G,Lovell JF,Jin H,Yang K.USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer.Theranostics.2020 Jul 23;10(20):9332-9347.doi:10.7150/thno.47137.PMID:32802195;PMCID:PMC7415808.
Species: Mouse
A02236-3 usage in article: APP:IF, SAMPLE:LUNG TISSUE, DILUTION:NA

Jia-Hui Yan,Yi-Shun Ji,Man-Li Yang,Jun Fu,Hu Shan,Li−Li Wang,Lei Zhang,Jing-Yuan Xiao,Xiao-Ying Kong, and Jin-Sheng Shi ACS Applied Nano Materials 2020 3 (9), 8817-8828 DOI: 10.1021/acsanm.0c01607
Species: Cuttlefish,Human,Mouse
A02236-3 usage in article: APP:IHC, SAMPLE:HEART,LIVER,SPLEEN,LUNG AND KIDNEY, DILUTION:NA

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SKU:A02236-3

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A02236-3
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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