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Cd8a antibody (A02236-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of mouse spleen tissues using anti-CD8 alpha/Cd8a antibody (A02236-3).
Overlay histogram showing mouse spleen tissues stained with A02236-3 (Blue line).The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD8 alpha/Cd8a Antibody (A02236-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing mouse spleen tissues stained with A02236-3 (Blue line).The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD8 alpha/Cd8a Antibody (A02236-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD8 alpha/Cd8a Antibody Picoband®
Cd8a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Cd8a antibody was positively detected by WB in:
rat thymus tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cd8a antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Cd8a antibody was positively detected by FC in:
mouse spleen tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
FC
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Tested
Product Information
Product Name
Anti-CD8 alpha/Cd8a Antibody Picoband®
View all CD8A Antibodies
View all CD8A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD8 recombinant protein (Position: K39-K245).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated Molecular Weight
27.5 kDa
Background of Cd8a
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Cite This Product
Anti-CD8 alpha/Cd8a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
69%
Low homology
Monkey
55%
Low homology
Human
54%
Low homology
Dog
53%
Low homology
Sheep
51%
Low homology
Cow
50%
Low homology
Rabbit
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cd8a antibody
Immunohistochemistry (IHC) protocol for Cd8a antibody
Flow Cytometry (FC) protocol for Cd8a antibody
Publications
Application:
Species:
Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment
Published: 2024-03-25
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: IF,IHC
Impact Factor: 4.20
Species: Mouse
PMID: 38526656
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation
Published: 2007-02-12
Journal: FERTILITY AND STERILITY
Application: IHC
Impact Factor: 3.28
Species: Rat
PMID: 17296191
Induction of immune tolerance with heart-thymus composite allotransplantation in rats
Published: 2005-10-01
Journal: JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY
Impact Factor: 0.19
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Product Promise
We promise Anti-CD8 alpha/Cd8a Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A02236-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026