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Product Info Summary
| SKU: | M01281 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CD81 Antibody
SKU/Catalog Number
M01281
Size
50 µl
Description
Boster Bio Anti-CD81 Antibody (Catalog # M01281). Tested in WB application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CD81 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01281)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1668CT270.93.9
Isotype
IgG1,k
Immunogen
This CD81 antibody is generated from a mouse immunized with recombinant protein from the human region of human CD81.
Reactive Species
M01281 is reactive to CD81 in Human, Rat
Calculated molecular weight
25.8 kDa
Background of CD81
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01281 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:1000
Validation Images & Assay Conditions
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Anti-CD81 Antibody at 1:4000 dilution + rat cerebellum whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CD81 Antibody at 1:4000 dilution + human testis whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CD81 Antibody at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Ramos whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human liver section using Pink1 (Cat#M01281). M01281 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-CD81 Antibody (M01281)
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