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Product Info Summary
| SKU: | PB9251 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD86 Antibody Picoband®
SKU/Catalog Number
PB9251
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD86 Antibody Picoband® catalog # PB9251. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD86 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9251)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9251 is reactive to CD86 in Human, Mouse
Observed Molecular Weight
60-80 kDa
Calculated molecular weight
37.7 kDa
Background of CD86
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9251 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml
Positive Control
WB: human Raji whole cell, human Daudi whole cell, mouse spleen tissue, mouse thymus tissue, mouse J774A1 whole cell, mouse Ana-1 whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of CD86 using anti-CD86 antibody (PB9251).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # PB9251) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.
Click image to see more details
Western blot analysis of CD86 using anti-CD86 antibody (PB9251).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse J774A.1 whole cell lysates,
Lane 4: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # PB9251) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.
Click image to see more details
IHC analysis of CD86 using anti-CD86 antibody (PB9251).
CD86 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD86 Antibody (PB9251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD86 Antibody Picoband® (PB9251)
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5 Customer Q&As for Anti-CD86 Antibody Picoband®
Question
My boss were happy with the WB result of your anti-CD86 antibody. However we have been able to see positive staining in foreskin cell membrane using this antibody. Is that expected? Could you tell me where is CD86 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
According to literature, foreskin does express CD86. Generally CD86 expresses in cell membrane. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-CD86 antibody PB9251 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The anti-CD86 antibody (PB9251) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-26
Question
We have seen staining in human foreskin. Any tips? Is anti-CD86 antibody supposed to stain foreskin positively?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Based on literature foreskin does express CD86. Based on Uniprot.org, CD86 is expressed in leukocyte, brain, foreskin, among other tissues. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2019-07-12
Question
We are interested in using your anti-CD86 antibody for negative regulation of t cell proliferation studies. Has this antibody been tested with western blotting on human raji whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
I appreciate your inquiry. This PB9251 anti-CD86 antibody is tested on human raji whole cell lysates, u937 cells. It is guaranteed to work for Flow Cytometry, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-04
Question
We ordered your anti-CD86 antibody for IHC-F on brain in a previous project. I am using human, and We want to use the antibody for WB next. My question regards examining brain as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?
H. Yang
Verified customer
Asked: 2018-12-14
Answer
I viewed the website and datasheets of our anti-CD86 antibody and it appears that PB9251 has been validated on human in both IHC-F and WB. Thus PB9251 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-14
