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Product Info Summary
| SKU: | M02982 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD97 Monoclonal Antibody
SKU/Catalog Number
M02982
BM5414 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD97 Monoclonal Antibody catalog # M02982. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD97 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02982)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADEF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD97 Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Reactive Species
M02982 is reactive to CD97 in Human, Mouse
Observed Molecular Weight
75 kDa
Calculated molecular weight
91.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02982 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human U251 whole cell, human PC-3 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CD97 using anti-CD97 antibody (M02982).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD97 antigen affinity purified monoclonal antibody (M02982) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD97 at approximately 75 kDa. The expected band size for CD97 is at 92 kDa.
Specific Publications For Anti-CD97 Monoclonal Antibody (M02982)
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5 Customer Q&As for Anti-CD97 Monoclonal Antibody
Question
Would M02982 anti-CD97 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It shows on the product datasheet, M02982 anti-CD97 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-CD97 Monoclonal antibody M02982 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-CD97 Monoclonal antibody (M02982) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
you antibody to test anti-CD97 Monoclonal antibody M02982 on mouse liver for research purposes, then I may be interested in using anti-CD97 Monoclonal antibody M02982 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The products we sell, including anti-CD97 Monoclonal antibody M02982, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-CD97 Monoclonal antibody M02982 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of liver, CD97 is highly expressed in liver. So, it is likely that anti-CD97 Monoclonal antibody M02982 will work for IF with liver.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-CD97 Monoclonal antibody (M02982)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
We do provide the blocking peptide for product anti-CD97 Monoclonal antibody (M02982). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-05
