Anti-CD98/Slc3a2 Antibody Picoband™

Product Name

Anti-CD98/Slc3a2 Antibody Picoband™

View all CD98 Antibodies

SKU/Catalog Number

A01794-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD98/Slc3a2 Antibody Picoband™ catalog # A01794-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CD98/Slc3a2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01794-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse CD98/Slc3a2 recombinant protein (Position: K35-Q497).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01794-3 is reactive to SLC3A2 in Mouse, Rat

Applications

A01794-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

80-90 kDa

Calculated molecular weight

67.994kDa

Background of CD98

4F2 cell-surface antigen heavy chain is a protein that in humans is encoded by the SLC3A2 (solute carrier family 3 member 2) gene. Predicted to enable amino acid transmembrane transporter activity and double-stranded RNA binding activity. Predicted to be involved in carboxylic acid transport and viral entry into host cell. Predicted to act upstream of or within response to exogenous dsRNA. Located in apical pole of neuron. Is active in synapse. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and spinal cord. Orthologous to human SLC3A2 (solute carrier family 3 member 2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/ml/1x10^6 cells, Mouse
Direct ELISA, 0.1-0.5 μg/ml/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CD98/Slc3a2 using anti-CD98/Slc3a2 antibody (A01794-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse pancreas tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD98/Slc3a2 antigen affinity purified polyclonal antibody (Catalog # A01794-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD98/Slc3a2 at approximately 80-90 kDa. The expected band size for CD98/Slc3a2 is at 68 kDa.

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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-CD98/Slc3a2 antibody (A01794-3).
Overlay histogram showing RAW264.7 cells stained with A01794-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD98/Slc3a2 Antibody (A01794-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLC3A2 (Source: Uniprot.org, NCBI)

Alternative Names

4F2; 4F2hc; 4T2HC; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC; CD98 antigen; CD98 heavy chain; CD98; CD98HC; CH98hc; FRP-1; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1antigen defined by monoclonal 4F2, heavy chain; monoclonal 44D7; NACAE4F2 cell-surface antigen heavy chain; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE solute carrier family 3 member 2 4F2 cell-surface antigen heavy chain|CD98 heavy chain|antigen defined by monoclonal antibody 4F2, heavy chain|antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43|lymphocyte activation antigen 4F2 large subunit|monoclonal antibody 44D7|solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2|solute carrier family 3 (amino acid transporter heavy chain), member 2

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