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Product Info Summary
| SKU: | A01367-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CDC45L Antibody Picoband®
SKU/Catalog Number
A01367-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDC45L Antibody Picoband® catalog # A01367-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC45L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01367-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CDC45L recombinant protein (Position: E166-A431).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01367-1 is reactive to CDC45 in Human, Mouse, Rat
Observed Molecular Weight
66 kDa
Calculated molecular weight
65.6 kDa
Background of CDC45
CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01367-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, rat thymus tissue, rat brain tissue, rat lung tissue, mouse testis tissue, mouse thymus tissue, mouse brain tissue, mouse lung tissue, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CDC45L using anti-CDC45L antibody (A01367-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC45L antigen affinity purified polyclonal antibody (Catalog # A01367-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
Specific Publications For Anti-CDC45L Antibody Picoband® (A01367-1)
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1 Customer Q&As for Anti-CDC45L Antibody Picoband®
Question
We are currently using anti-CDC45L antibody A01367-1 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
E. Kulkarni
Verified customer
Asked: 2014-03-12
Answer
The anti-CDC45L antibody (A01367-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-12
