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Product Info Summary
| SKU: | A13717-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDCA4 Antibody
SKU/Catalog Number
A13717-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDCA4 Antibody (Catalog# A13717-1). Tested in IHC, ICC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13717-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CDCA4. Immunogen sequence location: 121-170
Reactive Species
A13717-1 is reactive to CDCA4 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
26.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13717-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
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Immunofluorescence analysis of HepG2 cells, using CDCA4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDCA4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CDCA4 Antibody (A13717-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CDCA4 Antibody
Question
Is a blocking peptide available for product anti-CDCA4 antibody (A13717-1)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do provide the blocking peptide for product anti-CDCA4 antibody (A13717-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-21
Question
I am looking for to test anti-CDCA4 antibody A13717-1 on human female gonad for research purposes, then I may be interested in using anti-CDCA4 antibody A13717-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The products we sell, including anti-CDCA4 antibody A13717-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-CDCA4 antibody A13717-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
P. Edwards
Verified customer
Asked: 2019-12-27
Answer
The anti-CDCA4 antibody (A13717-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
I see that the anti-CDCA4 antibody A13717-1 works with IF, what is the protocol used to produce the result images on the product page?
R. Lewis
Verified customer
Asked: 2013-09-12
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-09-12
Question
Can you help my question with product A13717-1, anti-CDCA4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Li
Verified customer
Asked: 2013-09-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13717-1 anti-CDCA4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-05
