Click image to see more details
Product Info Summary
| SKU: | A13245 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-CDH24/Cadherin 24 Antibody
SKU/Catalog Number
A13245
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDH24/Cadherin 24 Antibody (Catalog# A13245). Tested in ICC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDH24/Cadherin 24 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13245)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CDH24. Immunogen sequence location: 81-130
Reactive Species
A13245 is reactive to CDH24 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
87.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13245 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using CDH24 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CDH24/Cadherin 24 Antibody (A13245)
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Customer Q&As
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7 Customer Q&As for Anti-CDH24/Cadherin 24 Antibody
Question
My question regarding product A13245, anti-CDH24/Cadherin 24 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13245 anti-CDH24/Cadherin 24 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-01
Question
Is a blocking peptide available for product anti-CDH24/Cadherin 24 antibody (A13245)?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
We do provide the blocking peptide for product anti-CDH24/Cadherin 24 antibody (A13245). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-07
Question
We are currently using anti-CDH24/Cadherin 24 antibody A13245 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-CDH24/Cadherin 24 antibody (A13245) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Would anti-CDH24/Cadherin 24 antibody A13245 work on monkey IF with cerebellar hemisphere?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Our lab technicians have not validated anti-CDH24/Cadherin 24 antibody A13245 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CDH24/Cadherin 24 antibody A13245 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellar hemisphere in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
See attached the WB image, lot number and protocol we used for testis using anti-CDH24/Cadherin 24 antibody A13245. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-11
Question
Is this A13245 anti-CDH24/Cadherin 24 antibody reactive to the isotypes of CDH24?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
The immunogen of A13245 anti-CDH24/Cadherin 24 antibody is A peptide derived from human CDH24. Immunogen sequence location: 81-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-28
Question
Does anti-CDH24/Cadherin 24 antibody A13245 work for IF with testis?
N. Brown
Verified customer
Asked: 2014-09-04
Answer
According to the expression profile of testis, CDH24 is highly expressed in testis. So, it is likely that anti-CDH24/Cadherin 24 antibody A13245 will work for IF with testis.
Boster Scientific Support
Answered: 2014-09-04
