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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IF, IHC, ICC, WB|
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Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Boster Bio Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody catalog # M00209-4. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00209-4)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human CDK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00209-4 is reactive to CDK1 in Human, Mouse, Rat
M00209-4 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (M00209-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-4) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Gene/Protein Information For CDK1 (Source: Uniprot.Org, NCBI)
Cyclin-dependent kinase 1
protein kinase superfamily
CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 188.8.131.52; EC 184.108.40.206; G1 to S and G2 to M; p34 protein kinase; P34CDC2 CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CDK1, check out the CDK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M00209-4 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
M00209-4 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:500
A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells
Human enterovirus 68 interferes with the host cell cycle to facilitate viral production
Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production
Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats
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