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Product Info Summary
| SKU: | A06507-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin-dependent kinase 10 Cdk10 Antibody
SKU/Catalog Number
A06507-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cyclin-dependent kinase 10 Cdk10 Antibody catalog # A06507-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin-dependent kinase 10 Cdk10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06507-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2B4
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Cdk10. at AA range: 30-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06507-1 is reactive to CDK10 in Human, Mouse, Rat
Applications
A06507-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.038kDa
Background of CDK10
Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using Cdk10 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Cdk10 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDK10 (Source: Uniprot.org, NCBI)
Gene Name
CDK10
Full Name
Cyclin-dependent kinase 10
Weight
41.038kDa
Superfamily
protein kinase superfamily
Alternative Names
Cell division protein kinase 10; cyclin-dependent kinase (CDC2-like) 10; cyclin-dependent kinase 10; cyclin-dependent kinase related protein; EC 2.7.11; EC 2.7.11.22; PISSLRECDC2-related protein kinase; serine/threonine protein kinase PISSLRE; Serine/threonine-protein kinase PISSLRE CDK10 ALSAS, PISSLRE cyclin dependent kinase 10 cyclin-dependent kinase 10|CDC2-related protein kinase|cell division protein kinase 10|cyclin-dependent kinase (CDC2-like) 10|cyclin-dependent kinase related protein|serine/threonine protein kinase PISSLRE
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK10, check out the CDK10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin-dependent kinase 10 Cdk10 Antibody (A06507-1)
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No publications found for A06507-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cyclin-dependent kinase 10 Cdk10 Antibody
Question
Would anti-Cdk10 antibody A06507-1 work for WB with pituitary gland?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
According to the expression profile of pituitary gland, CDK10 is highly expressed in pituitary gland. So, it is likely that anti-Cdk10 antibody A06507-1 will work for WB with pituitary gland.
Boster Scientific Support
Answered: 2019-09-20
Question
I was wanting to use your anti-Cdk10 antibody for WB for human pituitary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pituitary gland identification?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
You can see on the product datasheet, A06507-1 anti-Cdk10 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pituitary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
Can you help my question with product A06507-1, anti-Cdk10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06507-1 anti-Cdk10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A06507-1 anti-Cdk10 antibody reactive to the isotypes of CDK10?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The immunogen of A06507-1 anti-Cdk10 antibody is Synthesized peptide derived from the Internal region of human Cdk10. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-08
Question
Does A06507-1 anti-Cdk10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can see on the product datasheet, A06507-1 anti-Cdk10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-30
Question
We are currently using anti-Cdk10 antibody A06507-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
S. Parker
Verified customer
Asked: 2016-08-05
Answer
The anti-Cdk10 antibody (A06507-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-05
