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Product Info Summary
| SKU: | M00511 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-CDK5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00511
BM4276 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK5 Rabbit Monoclonal Antibody catalog # M00511. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00511)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdk5
Reactive Species
M00511 is reactive to CDK5 in Human, Mouse, Rat
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00511 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human SH-SY5Y whole cell, human PC-3 whole cell, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: Human esophageal carcinoma tissue, Rat stomach tissue, Human stomach tissue, Rat heart tissue, Human prostate cancer tissue, human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of CDK5 using anti-CDK5 antibody (M00511).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 antigen affinity purified monoclonal antibody (M00511) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDK5 at approximately 33 kDa. The expected band size for CDK5 is at 33 kDa.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using CDK5 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-CDK5 Rabbit Monoclonal Antibody (M00511)
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6 Customer Q&As for Anti-CDK5 Rabbit Monoclonal Antibody
Question
Does anti-CDK5 Rabbit Monoclonal antibody M00511 work on feline WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not tested anti-CDK5 Rabbit Monoclonal antibody M00511 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CDK5 Rabbit Monoclonal antibody M00511 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-CDK5 Rabbit Monoclonal antibody M00511 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-15
Question
Is this M00511 anti-CDK5 Rabbit Monoclonal antibody reactive to the isotypes of CDK5?
H. Krishna
Verified customer
Asked: 2019-09-10
Answer
The immunogen of M00511 anti-CDK5 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cdk5 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-10
Question
Is a blocking peptide available for product anti-CDK5 Rabbit Monoclonal antibody (M00511)?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We do provide the blocking peptide for product anti-CDK5 Rabbit Monoclonal antibody (M00511). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-CDK5 Rabbit Monoclonal antibody for IP for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
As indicated on the product datasheet, M00511 anti-CDK5 Rabbit Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-25
Question
I have a question about product M00511, anti-CDK5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Li
Verified customer
Asked: 2015-08-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00511 anti-CDK5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-24
