Product Info Summary
SKU: | A02973-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CDR2 Antibody Picoband™
SKU/Catalog Number
A02973-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDR2 Antibody Picoband™ catalog # A02973-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02973-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CDR2, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02973-1 is reactive to CDR2 in Human, Mouse
Applications
A02973-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.855kDa
Background of CDR2
Cerebellar degeneration-related protein 2 is a protein that in humans is encoded by the CDR2 gene. By a combination of study of rodent/human somatic cell hybrids and in situ hybridization, this gene is assigned to 16p13.1-p12. The onconeuronal cerebellar degeneration-related antigen Cdr2 is associated with paraneoplastic syndromes. Neoplastic expression of Cdr2 in ovary and breast tumors triggers an autoimmune response that suppresses tumor growth by developing tumor immunity, but culminates in cerebellar degeneration when Cdr2-specific immune cells recognize neuronal Cdr2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CDR2 using anti-CDR2 antibody (A02973-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse kidney tissue lysate,
lane 2: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR2 antigen affinity purified polyclonal antibody (Catalog # A02973-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR2 at approximately 52KD. The expected band size for CDR2 is at 52KD.
Protein Target Info & Infographic
Gene/Protein Information For CDR2 (Source: Uniprot.org, NCBI)
Gene Name
CDR2
Full Name
Cerebellar degeneration-related protein 2
Weight
51.855kDa
Superfamily
CDR2 family
Alternative Names
CDR62; cerebellar degeneration-related protein (62kD); cerebellar degeneration-related protein 2; cerebellar degeneration-related protein 2, 62kDa; Major Yo paraneoplastic antigen; Paraneoplastic cerebellar degeneration-associated antigen; PCD17; Yo paraneoplastic antigen; Yo CDR2 CDR62, Yo cerebellar degeneration related protein 2 cerebellar degeneration-related protein 2|Yo paraneoplastic antigen|cerebellar degeneration-related protein 2, 62kDa|major Yo paraneoplastic antigen|paraneoplastic cerebellar degeneration-associated antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDR2, check out the CDR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDR2 Antibody Picoband™ (A02973-1)
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No publications found for A02973-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CDR2 Antibody Picoband™
Question
I see that the anti-CDR2 antibody A02973-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-03
Question
Would A02973-1 anti-CDR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
As indicated on the product datasheet, A02973-1 anti-CDR2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-28
Question
We need to test anti-CDR2 antibody A02973-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-CDR2 antibody A02973-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The products we sell, including anti-CDR2 antibody A02973-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-CDR2 antibody A02973-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-31
Question
Would anti-CDR2 antibody A02973-1 work for WB with cervix carcinoma erythroleukemia?
S. Banerjee
Verified customer
Asked: 2019-05-20
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CDR2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-CDR2 antibody A02973-1 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-05-20
Question
Would anti-CDR2 antibody A02973-1 work on bovine WB with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
Our lab technicians have not tested anti-CDR2 antibody A02973-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CDR2 antibody A02973-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine pigmented layer of retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-31
Question
We are currently using anti-CDR2 antibody A02973-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
H. Baker
Verified customer
Asked: 2015-05-27
Answer
The anti-CDR2 antibody (A02973-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-27