Anti-CDR2 Antibody Picoband™

Product Name

Anti-CDR2 Antibody Picoband™

View all CDR2 Antibodies

SKU/Catalog Number

A02973-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDR2 Antibody Picoband™ catalog # A02973-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02973-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CDR2, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02973-1 is reactive to CDR2 in Human, Mouse

Applications

A02973-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

51.855kDa

Background of CDR2

Cerebellar degeneration-related protein 2 is a protein that in humans is encoded by the CDR2 gene. By a combination of study of rodent/human somatic cell hybrids and in situ hybridization, this gene is assigned to 16p13.1-p12. The onconeuronal cerebellar degeneration-related antigen Cdr2 is associated with paraneoplastic syndromes. Neoplastic expression of Cdr2 in ovary and breast tumors triggers an autoimmune response that suppresses tumor growth by developing tumor immunity, but culminates in cerebellar degeneration when Cdr2-specific immune cells recognize neuronal Cdr2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CDR2 using anti-CDR2 antibody (A02973-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse kidney tissue lysate,
lane 2: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR2 antigen affinity purified polyclonal antibody (Catalog # A02973-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR2 at approximately 52KD. The expected band size for CDR2 is at 52KD.

Gene/Protein Information For CDR2 (Source: Uniprot.org, NCBI)

Alternative Names

CDR62; cerebellar degeneration-related protein (62kD); cerebellar degeneration-related protein 2; cerebellar degeneration-related protein 2, 62kDa; Major Yo paraneoplastic antigen; Paraneoplastic cerebellar degeneration-associated antigen; PCD17; Yo paraneoplastic antigen; Yo CDR2 CDR62, Yo cerebellar degeneration related protein 2 cerebellar degeneration-related protein 2|Yo paraneoplastic antigen|cerebellar degeneration-related protein 2, 62kDa|major Yo paraneoplastic antigen|paraneoplastic cerebellar degeneration-associated antigen

For more info on CDR2, check out the CDR2 Infographic

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In this infographic, you will see the following information for CDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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