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Product Info Summary
| SKU: | M01035 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDT1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01035
BM5203 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDT1 Rabbit Monoclonal Antibody catalog # M01035. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01035)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDT1.
Reactive Species
M01035 is reactive to CDT1 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
60.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01035 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human 293T whole cell, human K562 whole cell, human Jurkat whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CDT1 using anti-CDT1 antibody (M01035).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1 antigen affinity purified monoclonal antibody (M01035) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDT1 at approximately 70 kDa. The expected band size for CDT1 is at 65 kDa.
Specific Publications For Anti-CDT1 Rabbit Monoclonal Antibody (M01035)
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4 Customer Q&As for Anti-CDT1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CDT1 Monoclonal antibody for IP for human embl:aah09410.1} on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embl:aah09410.1} identification?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
You can see on the product datasheet, M01035 anti-CDT1 Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embl:aah09410.1} in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
We are currently using anti-CDT1 Monoclonal antibody M01035 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
M. Brown
Verified customer
Asked: 2020-01-16
Answer
The anti-CDT1 Monoclonal antibody (M01035) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
Question
Is a blocking peptide available for product anti-CDT1 Monoclonal antibody (M01035)?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
We do provide the blocking peptide for product anti-CDT1 Monoclonal antibody (M01035). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-16
Question
Do you have a BSA free version of anti-CDT1 Monoclonal antibody M01035 available?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDT1 Monoclonal antibody M01035 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-06
