Anti-CEACAM1 (human) Monoclonal Antibody (GM8G5)

Product Name

Anti-CEACAM1 (human) Monoclonal Antibody (GM8G5)

View all CEACAM1 Antibodies

SKU/Catalog Number

M00923-1

Size

100µg

Form

Liquid. In PBS, pH 7.2. Contains no preservatives.

Description

Boster Bio Anti-CEACAM1 (human) Monoclonal Antibody (GM8G5) catalog # M00923-1. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEACAM1 (human) Monoclonal Antibody (GM8G5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00923-1)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2. Contains no preservatives.

Clonality

Monoclonal

Clone Number

GM8G5

Isotype

IgM, kappa

Immunogen

Vector containing the cDNA of human CEACAM1 A2 domain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00923-1 is reactive to CEACAM1 in Human

Applications

M00923-1 is guaranteed for Flow Cytometry, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

57.56kDa

Background of CEACAM1

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (M00923-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # M00923-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for CEACAM1.

Gene/Protein Information For CEACAM1 (Source: Uniprot.org, NCBI)

Alternative Names

Nothing Found CEACAM1 BGP, BGP1, BGPI CEA cell adhesion molecule 1 carcinoembryonic antigen-related cell adhesion molecule 1|CD66a antigen|antigen CD66|carcinoembryonic antigen related cell adhesion molecule 1|carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)

For more info on CEACAM1, check out the CEACAM1 Infographic

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In this infographic, you will see the following information for CEACAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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