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Product Info Summary
| SKU: | M03197-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CEACAM6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03197-1
BM5223 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEACAM6 Rabbit Monoclonal Antibody catalog # M03197-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEACAM6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03197-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACBC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEACAM6
Reactive Species
M03197-1 is reactive to CEACAM6 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
37.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03197-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB:rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of CEACAM6 using anti-CEACAM6 antibody (M03197-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM6 antigen affinity purified monoclonal antibody (M03197-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CEACAM6 at approximately 60 kDa. The expected band size for CEACAM6 is at 37 kDa.
Specific Publications For Anti-CEACAM6 Rabbit Monoclonal Antibody (M03197-1)
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5 Customer Q&As for Anti-CEACAM6 Rabbit Monoclonal Antibody
Question
Would anti-CEACAM6 Monoclonal antibody M03197-1 work for WB with upper lobe of lung?
G. Miller
Verified customer
Asked: 2019-11-20
Answer
According to the expression profile of upper lobe of lung, CEACAM6 is highly expressed in upper lobe of lung. So, it is likely that anti-CEACAM6 Monoclonal antibody M03197-1 will work for WB with upper lobe of lung.
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-CEACAM6 Monoclonal antibody for WB for human upper lobe of lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human upper lobe of lung identification?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
You can see on the product datasheet, M03197-1 anti-CEACAM6 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human upper lobe of lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-15
Question
My lab would like to test anti-CEACAM6 Monoclonal antibody M03197-1 on human upper lobe of lung for research purposes, then I may be interested in using anti-CEACAM6 Monoclonal antibody M03197-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Miller
Verified customer
Asked: 2016-09-28
Answer
The products we sell, including anti-CEACAM6 Monoclonal antibody M03197-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-09-28
Question
We are currently using anti-CEACAM6 Monoclonal antibody M03197-1 for rat tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
R. Bhatt
Verified customer
Asked: 2016-05-16
Answer
The anti-CEACAM6 Monoclonal antibody (M03197-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-16
Question
Is there a BSA free version of anti-CEACAM6 Monoclonal antibody M03197-1 available?
R. Miller
Verified customer
Asked: 2015-01-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CEACAM6 Monoclonal antibody M03197-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-23
