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Product Info Summary
|Reactive Species:||Human, Rat|
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Anti-CENPA Antibody Picoband™
Boster Bio Anti-CENPA Antibody Picoband™ catalog # PB9538. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CENPA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9538)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
PB9538 is reactive to CENPA in Human, Rat
PB9538 is guaranteed for WB Boster Guarantee
Background of CENPA
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. And CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. In higher eukaryotes, the recruitment of CENP-A nucleosomes to existing centromeres is an epigenetic process, independent of the underlying DNA sequence. In S.pombe, de novo recruitment of the CENP-A to the centromere is believed to be controlled by "centromeric" heterochromatin surrounding the centromere, and by an RNAi mechanism. The RNAi is cut to form siRNA; this complexes with the protein Chp1, which then binds the centromeric heterochromatin. This helps recruit other proteins, ultimately resulting in a protein complex that forms cohesin between two sister chromatids at the centromeric heterochromatin. This cohesin is believed to be essential in replacing the centromere H3 with CENP-A. CENP-A is one of the epigenetic changes that is believed to distinguish centromeric DNA from other DNA. Once the CENP-A has been added, the centromere becomes self-propagating, and the surrounding heterochromatin/RNAi mechanism is no longer necessary.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CENPA using anti-CENPA antibody (PB9538).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Rat Lung Tissue Lysate at 50ug,
Lane 3: Rat Pancreas Tissue Lysate at 50ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPA antigen affinity purified polyclonal antibody (Catalog # PB9538) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPA at approximately 16 kDa. The expected band size for CENPA is at 16 kDa.
Gene/Protein Information For CENPA (Source: Uniprot.org, NCBI)
Histone H3-like centromeric protein A
histone H3 family
Centromere autoantigen A; centromere protein A (17kD); centromere protein ACENP-Acentromere protein A, 17kDa; histone H3-like centromeric protein A CENPA CENP-A, CenH3 centromere protein A histone H3-like centromeric protein A|centromere autoantigen A|centromere protein A, 17kDa|centromere-specific histone*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CENPA, check out the CENPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CENPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for PB9538
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