Product Info Summary
| SKU: | A01894-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Centrosomal protein of 290 kDa CEP290 Antibody
SKU/Catalog Number
A01894-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Centrosomal protein of 290 kDa CEP290 Antibody catalog # A01894-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Centrosomal protein of 290 kDa CEP290 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01894-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CEP290. AA range:771-820
Reactive Species
A01894-1 is reactive to CEP290 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
290.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01894-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using CEP290 Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from K562 cells, using CEP290 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Centrosomal protein of 290 kDa CEP290 Antibody (A01894-1)
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Customer Q&As
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3 Customer Q&As for Anti-Centrosomal protein of 290 kDa CEP290 Antibody
Question
Is this A01894-1 anti-CEP290 antibody reactive to the isotypes of CEP290?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The immunogen of A01894-1 anti-CEP290 antibody is Synthesized peptide derived from the Internal region of human CEP290. at AA rangle: 740-820. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-18
Question
See below the WB image, lot number and protocol we used for brain using anti-CEP290 antibody A01894-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
Can you help my question with product A01894-1, anti-CEP290 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Gonzalez
Verified customer
Asked: 2015-03-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01894-1 anti-CEP290 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-10

