Click image to see more details
Product Info Summary
| SKU: | A30607 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-CEP350 Antibody
SKU/Catalog Number
A30607
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CEP350 Antibody (Catalog# A30607). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEP350 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30607)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CEP35. Immunogen sequence location: 671-720
Reactive Species
A30607 is reactive to CEP350 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
350.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30607 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CEP350 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CEP350 Antibody (A30607)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CEP350 Antibody
Question
I have attached the WB image, lot number and protocol we used for lymphoblast using anti-CEP350 antibody A30607. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
Will A30607 anti-CEP350 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
As indicated on the product datasheet, A30607 anti-CEP350 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-CEP350 antibody A30607 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The anti-CEP350 antibody (A30607) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-01
Question
I have a question about product A30607, anti-CEP350 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30607 anti-CEP350 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-27
Question
Will anti-CEP350 antibody A30607 work on goat IHC with lymphoblast?
J. Rodriguez
Verified customer
Asked: 2017-01-16
Answer
Our lab technicians have not validated anti-CEP350 antibody A30607 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CEP350 antibody A30607 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lymphoblast in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-16
