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Product Info Summary
| SKU: | A01661-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CEP63 Antibody Picoband®
SKU/Catalog Number
A01661-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-CEP63 Antibody Picoband® catalog # A00722-6. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CEP63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01661-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human CEP63 recombinant protein (Position: Q294-D628). Human CEP63 shares 76.4% amino acid (aa) sequence identity with mouse CEP63.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01661-1 is reactive to CEP63 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
81.3 kDa
Background of CEP63
Centrosomal protein of 63 kDa is a protein that in humans is encoded by the CEP63 gene. This gene encodes a protein with six coiled-coil domains. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. Several alternatively spliced transcript variants have been found, but their biological validity has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01661-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Western blot analysis of CEP63 using anti-CEP63 antibody (A01661-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP63 antigen affinity purified polyclonal antibody (Catalog # A01661-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP63 at approximately 70 kDa. The expected band size for CEP63 is at 81 kDa.
Specific Publications For Anti-CEP63 Antibody Picoband® (A01661-1)
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