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Product Info Summary
| SKU: | PB9852 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Ceruloplasmin/CP Antibody Picoband®
SKU/Catalog Number
PB9852
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ceruloplasmin/CP Antibody Picoband® catalog # PB9852. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ceruloplasmin/CP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9852)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Ceruloplasmin recombinant protein (Position: K20-M259). Human Ceruloplasmin shares 80.8% and 79.6% amino acid (aa) sequence identity with mouse and rat Ceruloplasmin, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9852 is reactive to CP in Human
Observed Molecular Weight
122 kDa
Calculated molecular weight
122.2 kDa
Background of CP
Ceruloplasmin (or caeruloplasmin) is a ferroxidase enzyme that in humans is encoded by the CP gene. It is mapped to 3q23-q25. The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe (II)transferrin to Fe (III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9852 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of Ceruloplasmin using anti-Ceruloplasmin antibody (PB9852).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin antigen affinity purified polyclonal antibody (PB9852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Ceruloplasmin at approximately 122 kDa. The expected band size for Ceruloplasmin is at 122 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-Ceruloplasmin antibody (PB9852).
Overlay histogram showing HepG2 cells stained with PB9852 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ceruloplasmin Antibody (PB9852, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Ceruloplasmin/CP Antibody Picoband® (PB9852)
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Customer Q&As
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16 Customer Q&As for Anti-Ceruloplasmin/CP Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Ceruloplasmin/CP antibody PB9852. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
We have tried in the past anti-Ceruloplasmin/CP antibody for IHC on plasma in the past. I am using human, and We intend to use the antibody for WB next. My lab would like examining plasma as well as cerebrospinal fluid in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
I viewed the website and datasheets of our anti-Ceruloplasmin/CP antibody and it seems that PB9852 has been tested on human in both IHC and WB. Thus PB9852 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-20
Question
Is a blocking peptide available for product anti-Ceruloplasmin/CP antibody (PB9852)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We do provide the blocking peptide for product anti-Ceruloplasmin/CP antibody (PB9852). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-24
Question
Our lab want to know about to test anti-Ceruloplasmin/CP antibody PB9852 on human plasma for research purposes, then I may be interested in using anti-Ceruloplasmin/CP antibody PB9852 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The products we sell, including anti-Ceruloplasmin/CP antibody PB9852, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this PB9852 anti-Ceruloplasmin/CP antibody reactive to the isotypes of CP?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The immunogen of PB9852 anti-Ceruloplasmin/CP antibody is E. coli-derived human Ceruloplasmin recombinant protein (Position: K20-M259). Human Ceruloplasmin shares 80.8% and 79.6% amino acid (aa) sequence identity with mouse and rat Ceruloplasmin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-18
Question
Please see the WB image, lot number and protocol we used for plasma using anti-Ceruloplasmin/CP antibody PB9852. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Will PB9852 anti-Ceruloplasmin/CP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
You can see on the product datasheet, PB9852 anti-Ceruloplasmin/CP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-25
Question
I see that the anti-Ceruloplasmin/CP antibody PB9852 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-Ceruloplasmin/CP antibody PB9852 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The anti-Ceruloplasmin/CP antibody (PB9852) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-11
Question
I am looking for using your anti-Ceruloplasmin/CP antibody for cellular protein metabolic process studies. Has this antibody been tested with western blotting on 22rv1 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
Thank you for your inquiry. This PB9852 anti-Ceruloplasmin/CP antibody is tested on 22rv1 whole cell lysates, a549 whole cell lysates. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-26
Question
I have a question about product PB9852, anti-Ceruloplasmin/CP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Mangal
Verified customer
Asked: 2018-06-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9852 anti-Ceruloplasmin/CP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-07
Question
We have seen staining in human plasma. Any tips? Is anti-Ceruloplasmin/CP antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
According to literature plasma does express CP. According to Uniprot.org, CP is expressed in liver, bile, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have CP expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Boster Scientific Support
Answered: 2018-01-05
Question
Do you have a BSA free version of anti-Ceruloplasmin/CP antibody PB9852 available?
W. Johnson
Verified customer
Asked: 2017-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Ceruloplasmin/CP antibody PB9852 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-24
Question
I was wanting to use your anti-Ceruloplasmin/CP antibody for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?
E. Huang
Verified customer
Asked: 2015-10-21
Answer
You can see on the product datasheet, PB9852 anti-Ceruloplasmin/CP antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-21
Question
Does anti-Ceruloplasmin/CP antibody PB9852 work for WB with plasma?
F. Johnson
Verified customer
Asked: 2014-06-05
Answer
According to the expression profile of plasma, CP is highly expressed in plasma. So, it is likely that anti-Ceruloplasmin/CP antibody PB9852 will work for WB with plasma.
Boster Scientific Support
Answered: 2014-06-05
Question
My boss were content with the WB result of your anti-Ceruloplasmin/CP antibody. However we have observed positive staining in liver secreted. note=colocalizes with gcp1 in using this antibody. Is that expected? Could you tell me where is CP supposed to be expressed?
D. Moore
Verified customer
Asked: 2013-06-13
Answer
From what I have seen in literature, liver does express CP. Generally CP expresses in secreted. note=colocalizes with gcp1 in. Regarding which tissues have CP expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Boster Scientific Support
Answered: 2013-06-13
