Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody

Chordin-like 1/CHRDL1 antibody

Boster Bio Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody catalog # A08014. Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: A08014
Size: 100μl
Reactive Species: Human, Rat
Host: Mouse
Application: WB

Product Name

Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody

View all Chordin-like 1/CHRDL1 Antibodies

SKU/Catalog Number

A08014

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody catalog # A08014. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08014)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

1A3

Isotype

IgG

Immunogen

Purified recombinant human CHRDL1 protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08014 is reactive to Chrdl1 in Human, Rat

Applications

A08014 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

50.732kDa

Background of Chordin-like 1/CHRDL1

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Feng Cong, et,al. (1999) Mol. Cell. Biol; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Chrdl1 (Source: Uniprot.org, NCBI)

Gene Name

Chrdl1

Full Name

Chordin-like protein 1

Weight

50.732kDa

Alternative Names

CHL; Chordin-like 1; chordin-like protein 1; CHRDL1; CRDL1; dA141H5.1; Neuralin 1; neuralin-1; neurogenesin-1; NRLN1; NRLN1neuralin 1; Ventroptin alpha; ventroptin; VOPT Chrdl1|CHL, CHL1, N, Nrln1, V, VOPT|chordin-like 1|chordin-like protein 1|neuralin-1|neurogenesin-1|ventroptin-alpha|ventroptin-beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Chrdl1, check out the Chrdl1 Infographic

Chrdl1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Chrdl1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-CHRDL1/Chordin Like 1 Monoclonal Antibody

Question

We are currently using anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

The anti-CHRDL1/Chordin Like 1 Monoclonal antibody (A08014) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-14

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-18

Question

I have attached the WB image, lot number and protocol we used for adipose tissue using anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-27

Question

Will anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014 work on bovine WB with astrocyte?

G. Anderson

Verified customer

Asked: 2015-01-06

Answer

Our lab technicians have not validated anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CHRDL1/Chordin Like 1 Monoclonal antibody A08014 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine astrocyte in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-01-06

Order DetailsPrice
A08014

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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