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Product Info Summary
| SKU: | M10029 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-CHST1 Antibody (C-term)
SKU/Catalog Number
M10029
Size
80 µl
Description
Boster Bio Anti-CHST1 Antibody (C-term) (Catalog # M10029). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CHST1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10029)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CHST1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 392-426 amino acids from the C-terminal region of human CHST1.
Reactive Species
M10029 is reactive to CHST1 in Human, Mouse, Rat
Calculated molecular weight
46.7 kDa
Background of CHST1
Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10029 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-CHST1 Antibody (C-term) at 1:2000 dilution
Lane 1: human brain lysates
Lane 2: Hela whole cell lysates
Lane 3: Jurkat whole cell lysates
Lane 4: mouse brain lysates
Lane 5: rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M10029 staining CHST1 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-CHST1 Antibody (C-term) (M10029)
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