Anti-CHST2 Antibody

Product Name

Anti-CHST2 Antibody

View all Carbohydrate Sulfotransferase 2/CHST2 Antibodies

SKU/Catalog Number

A08119

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CHST2 Antibody (Catalog # A08119). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CHST2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08119)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CHST2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08119 is reactive to CHST2 in Human

Applications

A08119 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.857kDa

Background of Carbohydrate Sulfotransferase 2/CHST2

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.

Uchimura K., J. Biochem. 124:670-678(1998).
Li X., Genomics 55:345-347(1999).
Sakaguchi H., Biochim. Biophys. Acta 1523:269-276(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from Jurkat cells, A549 cells and COLO cells, using CHST2 antibody A08119.

Gene/Protein Information For CHST2 (Source: Uniprot.org, NCBI)

Alternative Names

C6ST; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2; Carbohydrate Sulfotransferase 2; CHST2; EC 2.8.2.-; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-1; GN6ST; Gn6ST-1; GST2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1 CHST2 C6ST, GST-2, GST2, Gn6ST-1, HEL-S-75, glcNAc6ST-1 carbohydrate sulfotransferase 2 carbohydrate sulfotransferase 2|N-acetylglucosamine 6-O-sulfotransferase 1|carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2|carbohydrate (chondroitin 6/keratan) sulfotransferase 2|epididymis secretory protein Li 75|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2

For more info on CHST2, check out the CHST2 Infographic

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In this infographic, you will see the following information for CHST2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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