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Product Info Summary
| SKU: | PB9527 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-cIAP2/BIRC3 Antibody Picoband®
SKU/Catalog Number
PB9527
PB0567 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-cIAP2/BIRC3 Antibody Picoband® catalog # PB9527. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-cIAP2/BIRC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9527)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human cIAP2 recombinant protein (Position: M1-K191). Human cIAP2 shares 69.8% amino acid (aa) sequence identity with mouse cIAP2.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9527 is reactive to BIRC3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.4 kDa
Background of BIRC3
Baculoviral IAP repeat-containing protein 3 (also known as cIAP2) is a protein that in humans is encoded by the BIRC3 gene. cIAP2 is a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9527 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HEPG2 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of cIAP2 using anti-cIAP2 antibody (PB9527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPG2 Whole Cell Lysate at 40ug,
Lane 2: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP2 antigen affinity purified polyclonal antibody (Catalog # PB9527) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP2 at approximately 68 kDa. The expected band size for cIAP2 is at 68 kDa.
Specific Publications For Anti-cIAP2/BIRC3 Antibody Picoband® (PB9527)
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1 Customer Q&As for Anti-cIAP2/BIRC3 Antibody Picoband®
Question
We are currently using anti-cIAP2/BIRC3 antibody PB9527 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The anti-cIAP2/BIRC3 antibody (PB9527) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
