Product Info Summary
SKU: | PA1786 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CISH Antibody
View all CISH/CIS-1 Antibodies
SKU/Catalog Number
PA1786
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CISH Antibody catalog # PA1786. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CISH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1786)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CISH, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1786 is reactive to CISH in Human, Mouse, Rat
Applications
PA1786 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
28.663kDa
Background of CISH/CIS-1
CISH (cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling (SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-CISH antibody, PA1786, Western blotting
All lanes: Anti CISH (PA1786) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
Click image to see more details
Anti-CISH antibody, PA1786, IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-CISH antibody, PA1786, IHC(P)
IHC(P): Rat Kidney Tissue
Protein Target Info & Infographic
Gene/Protein Information For CISH (Source: Uniprot.org, NCBI)
Gene Name
CISH
Full Name
Cytokine-inducible SH2-containing protein
Weight
28.663kDa
Alternative Names
CIS-1; CIS-1cytokine-inducible SH2-containing protein; CIScytokine-inducible inhibitor of signaling type 1B; CISH; cytokine inducible SH2-containing protein; G18Protein G18; SOCS; Suppressor of cytokine signaling CISH BACTS2, CIS, CIS-1, G18, SOCS cytokine inducible SH2 containing protein cytokine-inducible SH2-containing protein|cytokine-inducible inhibitor of signaling type 1B|suppressor of cytokine signaling
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CISH, check out the CISH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CISH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CISH Antibody (PA1786)
Hello CJ!
No publications found for PA1786
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CISH Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-CISH Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-CISH Antibody
Question
We are currently using anti-CISH antibody PA1786 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
E. Gonzalez
Verified customer
Asked: 2019-11-27
Answer
The anti-CISH antibody (PA1786) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-27
Question
Is a blocking peptide available for product anti-CISH antibody (PA1786)?
K. Krishna
Verified customer
Asked: 2019-06-20
Answer
We do provide the blocking peptide for product anti-CISH antibody (PA1786). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-CISH antibody PA1786. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-07
Question
I see that the anti-CISH antibody PA1786 works with WB, what is the protocol used to produce the result images on the product page?
D. Li
Verified customer
Asked: 2015-02-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-02-13