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Product Info Summary
| SKU: | M02966 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CITED2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02966
BM4825 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CITED2 Rabbit Monoclonal Antibody catalog # M02966. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CITED2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02966)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IEA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CITED2
Reactive Species
M02966 is reactive to CITED2 in Human, Mouse
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02966 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of CITED2 expression in HeLa cell lysate.
Specific Publications For Anti-CITED2 Rabbit Monoclonal Antibody (M02966)
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6 Customer Q&As for Anti-CITED2 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-CITED2 Rabbit Monoclonal antibody (M02966)?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
We do provide the blocking peptide for product anti-CITED2 Rabbit Monoclonal antibody (M02966). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-20
Question
I would like to test anti-CITED2 Rabbit Monoclonal antibody M02966 on human female gonad for research purposes, then I may be interested in using anti-CITED2 Rabbit Monoclonal antibody M02966 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The products we sell, including anti-CITED2 Rabbit Monoclonal antibody M02966, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-18
Question
We are currently using anti-CITED2 Rabbit Monoclonal antibody M02966 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
N. Zhang
Verified customer
Asked: 2018-06-26
Answer
The anti-CITED2 Rabbit Monoclonal antibody (M02966) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-26
Question
Would M02966 anti-CITED2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Moore
Verified customer
Asked: 2017-10-04
Answer
It shows on the product datasheet, M02966 anti-CITED2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-04
Question
Can you help my question with product M02966, anti-CITED2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Wu
Verified customer
Asked: 2017-01-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02966 anti-CITED2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-12
Question
Will anti-CITED2 Rabbit Monoclonal antibody M02966 work on primate WB with pancreas?
G. Baker
Verified customer
Asked: 2016-08-30
Answer
Our lab technicians have not validated anti-CITED2 Rabbit Monoclonal antibody M02966 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CITED2 Rabbit Monoclonal antibody M02966 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-30
