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- Table of Contents
KRT7 antibody (A02416-2) performs well in IF, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IF analysis of KRT7 using anti-KRT7 antibody (A02416-2).
KRT7 was detected in a paraffin-embedded section of human throid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
KRT7 was detected in a paraffin-embedded section of human throid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-KRT7 Antibody Picoband®
KRT7 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, IHC, WB |
Tested Applications
KRT7 antibody was positively detected by WB in:
human Hela whole cell, human RT4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT7 antibody was positively detected by IHC in:
human breast cancer tissue, human thyroid cancer tissue, human placenta tissue, human stomach cancer tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT7 antibody was positively detected by IF in:
human throid cancer tissue, human stomach cancer tissue, human lung cancer tissue, human breast cancer tissue, human ovarian cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-KRT7 Antibody Picoband®
View all KRT7 Antibodies
View all KRT7 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KRT7, which shares 91.3% amino acid (aa) sequence identity with mouse and rat KRT7.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
51 kDa
Calculated Molecular Weight
51.4 kDa
Background of KRT7
Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Cite This Product
Anti-KRT7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02416-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
91%
High Homology
Rat
91%
High Homology
Cow
91%
High Homology
Sheep
91%
High Homology
Monkey
91%
High Homology
Rabbit
78%
Moderate Homology
Dog
78%
Moderate Homology
Zebrafish
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KRT7 antibody
Immunohistochemistry (IHC) protocol for KRT7 antibody
Immunofluorescence (IF) protocol for KRT7 antibody
Publications
Application:
Species:
Necroptosis-Related Prognostic Model for Pancreatic Carcinoma Reveals Its Invasion and Metastasis Potential through Hybrid EMT and Immune Escape
Published: 2023-06-16
Journal: Biomedicines
Application: IHC
Impact Factor: 4.70
Species: Human
PMID: 37371833
Infection of Placental Extravillous Cytotrophoblasts with Human Cytomegalovirus Causes a Treg/Th17 Imbalance at the Maternal–Fetal Interface
Published: 2020-07-22
Journal: CELL TRANSPLANTATION
Application: IHC
Impact Factor: 3.34
Species: Human
PMID: 32693638
LncRNA MEG3 inhibits trophoblast invasion and trophoblast-mediated VSMC loss in uterine spiral artery remodeling
Published: 2019-05-08
Journal: MOLECULAR REPRODUCTION AND DEVELOPMENT
Application: IHC
Impact Factor: 2.12
Species: Human
PMID: 31066488
Is Atrial Natriuretic Peptide (ANP) and Natriuretic Peptide Receptor-A (NPR-A) Expression in Human Placenta and Decidua Normal?
Published: 2019-04-19
Journal: MEDICAL SCIENCE MONITOR
Application: IHC
Impact Factor: 1.98
Species: Human
PMID: 31000687
Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer
Published: 2017-11-06
Journal: BIOSCIENCE REPORTS
Application: IHC
Impact Factor: 2.91
Species: Human
PMID: 28827446
Clinical and prognostic role of annexin A2 in adamantinomatous craniopharyngioma
Published: 2016-09-17
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: WB
Impact Factor: 2.75
Species: Human
PMID: 27640198
Ephrin-B2 mediates trophoblast-dependent maternal spiral artery remodeling in first trimester
Published: 2015-02-21
Journal: PLACENTA
Application: IHC
Impact Factor: 2.71
Species: Human
PMID: 25748802
Expression of RGC32 in human normal and preeclamptic placentas and its role in trophoblast cell invasion and migration
Published: 2015-01-10
Journal: PLACENTA
Application: ICC
Impact Factor: 2.71
Species: Human
PMID: 25655049
All-trans retinoic acid inhibits craniopharyngioma cell growth: study on an explant cell model
Published: 2013-02-20
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: IHC
Impact Factor: 3.12
Species: Human
PMID: 23423514
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Product Promise
We promise Anti-KRT7 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02416-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026