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Product Info Summary
| SKU: | A30466 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CKI-gamma2 Antibody
SKU/Catalog Number
A30466
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CKI-gamma2 Antibody (Catalog# A30466). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CKI-gamma2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30466)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CKI-gamma2. Immunogen sequence location: 10-59
Reactive Species
A30466 is reactive to CSNK1G2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
47.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30466 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of HeLa cells using Casein Kinase Iγ2 Polyclonal Antibody
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Western blot analysis of lysates from HeLa and 293 cells, using CKI-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CKI-gamma2 Antibody (A30466)
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Customer Q&As
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6 Customer Q&As for Anti-CKI-gamma2 Antibody
Question
We are currently using anti-CKI-gamma2 antibody A30466 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-CKI-gamma2 antibody (A30466) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use your anti-CKI-gamma2 antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
It shows on the product datasheet, A30466 anti-CKI-gamma2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-CKI-gamma2 antibody (A30466)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We do provide the blocking peptide for product anti-CKI-gamma2 antibody (A30466). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CKI-gamma2 antibody A30466. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Does A30466 anti-CKI-gamma2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
You can see on the product datasheet, A30466 anti-CKI-gamma2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-18
Question
I see that the anti-CKI-gamma2 antibody A30466 works with WB, what is the protocol used to produce the result images on the product page?
O. Miller
Verified customer
Asked: 2014-02-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-02-13
