Click image to see more details
Product Info Summary
| SKU: | A30611 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-CKLF1 CMTM1 Antibody
SKU/Catalog Number
A30611
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CKLF1 CMTM1 Antibody (Catalog# A30611). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CKLF1 CMTM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30611)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CKLF1. Immunogen sequence location: 120-169
Reactive Species
A30611 is reactive to CMTM1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
18.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30611 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of MCF7 cells, using CKLF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CKLF1 CMTM1 Antibody (A30611)
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Customer Q&As
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7 Customer Q&As for Anti-CKLF1 CMTM1 Antibody
Question
We are currently using anti-CKLF1 antibody A30611 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-CKLF1 antibody (A30611) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
Is this A30611 anti-CKLF1 antibody reactive to the isotypes of CMTM1?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of A30611 anti-CKLF1 antibody is A peptide derived from human CKLF1. Immunogen sequence location: 120-169. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
I was wanting to use your anti-CKLF1 antibody for IF for human right testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right testis identification?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
As indicated on the product datasheet, A30611 anti-CKLF1 antibody has been validated for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human right testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-29
Question
My question regarding product A30611, anti-CKLF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30611 anti-CKLF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-08
Question
Does A30611 anti-CKLF1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Bhatt
Verified customer
Asked: 2018-09-27
Answer
It shows on the product datasheet, A30611 anti-CKLF1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-27
Question
Is there a BSA free version of anti-CKLF1 antibody A30611 available?
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CKLF1 antibody A30611 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-CKLF1 antibody A30611. Let me know if you need anything else.
A. Baker
Verified customer
Asked: 2015-12-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-03
