Click image to see more details
Product Info Summary
| SKU: | A30616 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CLDN6/Claudin 6 Antibody
SKU/Catalog Number
A30616
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CLDN6/Claudin 6 Antibody (Catalog# A30616). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLDN6/Claudin 6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30616)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CLDN6. Immunogen sequence location: 81-130
Reactive Species
A30616 is reactive to CLDN6 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
23.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30616 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using Claudin-6 Polyclonal Antibody
Click image to see more details
Immunofluorescence analysis of HUVEC cells, using CLDN6 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using CLDN6 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Western blot analysis of lysates from Jurkat cells, using CLDN6 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CLDN6/Claudin 6 Antibody (A30616)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CLDN6/Claudin 6 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CLDN6/Claudin 6 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-CLDN6/Claudin 6 Antibody
Question
Our team were well pleased with the WB result of your anti-CLDN6/Claudin 6 antibody. However we have observed positive staining in placenta cell junction using this antibody. Is that expected? Could you tell me where is CLDN6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to literature, placenta does express CLDN6. Generally CLDN6 expresses in cell junction, tight junction. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-28
Question
you antibody using your anti-CLDN6/Claudin 6 antibody for cell-cell junction organization studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
I appreciate your inquiry. This A30616 anti-CLDN6/Claudin 6 antibody is tested on human ovary tissue, huvec cells. It is guaranteed to work for IF, IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-07
Question
We are currently using anti-CLDN6/Claudin 6 antibody A30616 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
The anti-CLDN6/Claudin 6 antibody (A30616) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-22
Question
We have been able to see staining in human placenta. Are there any suggestions? Is anti-CLDN6/Claudin 6 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
From what I have seen in literature placenta does express CLDN6. From what I have seen in Uniprot.org, CLDN6 is expressed in female gonad, placenta, among other tissues. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-28
Question
We ordered your anti-CLDN6/Claudin 6 antibody for IF on placenta last year. I am using human, and We are going to use the antibody for IHC next. We are interested in examining placenta as well as female gonad in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
A. Jha
Verified customer
Asked: 2016-01-26
Answer
I looked at the website and datasheets of our anti-CLDN6/Claudin 6 antibody and it seems that A30616 has been tested on human in both IF and IHC. Thus A30616 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-01-26
