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Product Info Summary
| SKU: | A11153 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cleaved-MMP-27 (Y99) Antibody
SKU/Catalog Number
A11153
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-MMP-27 (Y99) Antibody catalog # A11153. Tested in WB, ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-MMP-27 (Y99) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11153)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP27. AA range:80-129
Reactive Species
A11153 is reactive to MMP27 in Human, Monkey
Calculated molecular weight
59.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11153 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cleaved-MMP-27 (Y99) Polyclonal Antibody
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Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using MMP27 (Cleaved-Tyr99) Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cleaved-MMP-27 (Y99) Antibody (A11153)
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Customer Q&As
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3 Customer Q&As for Anti-Cleaved-MMP-27 (Y99) Antibody
Question
We are currently using anti-Cleaved-MMP-27 (Y99) antibody A11153 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The anti-Cleaved-MMP-27 (Y99) antibody (A11153) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-20
Question
Does anti-Cleaved-MMP-27 (Y99) antibody A11153 work on zebrafish WB with minor salivary gland?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not tested anti-Cleaved-MMP-27 (Y99) antibody A11153 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Cleaved-MMP-27 (Y99) antibody A11153 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish minor salivary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
I am interested in to test anti-Cleaved-MMP-27 (Y99) antibody A11153 on monkey minor salivary gland for research purposes, then I may be interested in using anti-Cleaved-MMP-27 (Y99) antibody A11153 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The products we sell, including anti-Cleaved-MMP-27 (Y99) antibody A11153, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-20
