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Product Info Summary
| SKU: | M06084 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB, FACS |
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Product info
Product Name
Anti-CLEC4E Antibody (Monoclonal, ABM42E6)
SKU/Catalog Number
M06084
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-CLEC4E Antibody (Monoclonal, ABM42E6) (Catalog# M06084). Tested in FACS, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-CLEC4E Antibody (Monoclonal, ABM42E6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06084)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM42C6
Isotype
IgG1 Kappa
Immunogen
A full length human CLEC4E protein
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M06084 is reactive to CLEC4E in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.1 kDa
Background of CLEC4E
This monoclonal antibody recognizes a 63kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06084 is guaranteed for WB, FACS Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Flow cytometry: 0.5-1 µg/10^6 cells
Validation Images & Assay Conditions
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Western blot analysis of CLEC4E. Anti-CLEC4E antibody (Clone: ABM42C6) was tested at 2 &mu
Specific Publications For Anti-CLEC4E Antibody (Monoclonal, ABM42E6) (M06084)
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1 Customer Q&As for Anti-CLEC4E Antibody (Monoclonal, ABM42E6)
Question
We are currently using anti-CLEC4E antibody (Monoclonal, ABM42E6) M06084 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
L. Johnson
Verified customer
Asked: 2013-05-10
Answer
The anti-CLEC4E antibody (Monoclonal, ABM42E6) (M06084) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-10
