Click image to see more details
Product Info Summary
| SKU: | A30546 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CNKR1 Antibody
SKU/Catalog Number
A30546
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CNKR1 Antibody (Catalog# A30546). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CNKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30546)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CNKR1. Immunogen sequence location: 491-540
Reactive Species
A30546 is reactive to CNKSR1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
79.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30546 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Click image to see more details
Western Blot analysis of COLO cells using CNK1 Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from COLO205 cells, using CNKR1 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Western blot analysis of the lysates from HT-29 cells using CNKSR1 antibody.
Specific Publications For Anti-CNKR1 Antibody (A30546)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CNKR1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CNKR1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-CNKR1 Antibody
Question
Is this A30546 anti-CNKR1 antibody reactive to the isotypes of CNKSR1?
T. Collins
Verified customer
Asked: 2020-04-13
Answer
The immunogen of A30546 anti-CNKR1 antibody is A peptide derived from human CNKR1. Immunogen sequence location: 491-540. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-13
Question
I have a question about product A30546, anti-CNKR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30546 anti-CNKR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-06
Question
I am looking for to test anti-CNKR1 antibody A30546 on mouse neuroepithelium for research purposes, then I may be interested in using anti-CNKR1 antibody A30546 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The products we sell, including anti-CNKR1 antibody A30546, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-02
Question
Would anti-CNKR1 antibody A30546 work on bovine WB with placenta?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Our lab technicians have not validated anti-CNKR1 antibody A30546 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CNKR1 antibody A30546 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-CNKR1 antibody A30546 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The anti-CNKR1 antibody (A30546) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-24
Question
I was wanting to use your anti-CNKR1 antibody for WB for mouse neuroepithelium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse neuroepithelium identification?
N. Johnson
Verified customer
Asked: 2016-04-20
Answer
As indicated on the product datasheet, A30546 anti-CNKR1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse neuroepithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-20
