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Product Info Summary
| SKU: | A07211-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CNT2 SLC28A2 Antibody
SKU/Catalog Number
A07211-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CNT2 SLC28A2 Antibody catalog # A07211-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CNT2 SLC28A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07211-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC28A2. AA range:371-420
Reactive Species
A07211-1 is reactive to SLC28A2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
71.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07211-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using CNT2 Polyclonal Antibody
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Western Blot analysis of 3T3 cells using CNT2 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using SLC28A2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using SLC28A2 antibody.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SLC28A2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CNT2 SLC28A2 Antibody (A07211-1)
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Customer Q&As
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7 Customer Q&As for Anti-CNT2 SLC28A2 Antibody
Question
My question regarding product A07211-1, anti-CNT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07211-1 anti-CNT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-13
Question
I was wanting to use to test anti-CNT2 antibody A07211-1 on human jejunal mucosa for research purposes, then I may be interested in using anti-CNT2 antibody A07211-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The products we sell, including anti-CNT2 antibody A07211-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for jejunal mucosa using anti-CNT2 antibody A07211-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Is a blocking peptide available for product anti-CNT2 antibody (A07211-1)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do provide the blocking peptide for product anti-CNT2 antibody (A07211-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-18
Question
Does anti-CNT2 antibody A07211-1 work for WB with jejunal mucosa?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
According to the expression profile of jejunal mucosa, SLC28A2 is highly expressed in jejunal mucosa. So, it is likely that anti-CNT2 antibody A07211-1 will work for WB with jejunal mucosa.
Boster Scientific Support
Answered: 2017-09-08
Question
Will A07211-1 anti-CNT2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Johnson
Verified customer
Asked: 2017-03-30
Answer
It shows on the product datasheet, A07211-1 anti-CNT2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-30
Question
We are currently using anti-CNT2 antibody A07211-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
D. Rodriguez
Verified customer
Asked: 2016-08-25
Answer
The anti-CNT2 antibody (A07211-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-25
