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Product Info Summary
| SKU: | A11407 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF |
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Product info
Product Name
Anti-CNTN5/Contactin 5 Antibody
View all Contactin-5 Antibodies
SKU/Catalog Number
A11407
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CNTN5/Contactin 5 Antibody (Catalog # A11407). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CNTN5/Contactin 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11407)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of humanCNTN5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11407 is reactive to CNTN5 in Human
Applications
A11407 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
120.686kDa
Background of Contactin-5
Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system By similarity.
Kamei Y., Genomics 69:113-119(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using CNTN5 antibody A11407.
Protein Target Info & Infographic
Gene/Protein Information For CNTN5 (Source: Uniprot.org, NCBI)
Gene Name
CNTN5
Full Name
Contactin-5
Weight
120.686kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CNTN5; contactin 5; Contactin5; Contactin-5; hNB-2; HNB-2s; MGC163491; NB2; NB-2; neural adhesion molecule; Neural recognition molecule NB-2 CNTN5 HNB-2s, NB-2 contactin 5 contactin-5|neural adhesion molecule|neural recognition molecule NB-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNTN5, check out the CNTN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNTN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CNTN5/Contactin 5 Antibody (A11407)
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No publications found for A11407
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CNTN5/Contactin 5 Antibody
Question
Does anti-CNTN5/Contactin 5 antibody A11407 work on horse IF with brain?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not tested anti-CNTN5/Contactin 5 antibody A11407 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CNTN5/Contactin 5 antibody A11407 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-CNTN5/Contactin 5 antibody A11407 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-05
Question
We are currently using anti-CNTN5/Contactin 5 antibody A11407 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The anti-CNTN5/Contactin 5 antibody (A11407) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-09
Question
Will A11407 anti-CNTN5/Contactin 5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
It shows on the product datasheet, A11407 anti-CNTN5/Contactin 5 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-21
Question
Does anti-CNTN5/Contactin 5 antibody A11407 work for IF with cerebellum?
M. Anderson
Verified customer
Asked: 2015-08-19
Answer
According to the expression profile of cerebellum, CNTN5 is highly expressed in cerebellum. So, it is likely that anti-CNTN5/Contactin 5 antibody A11407 will work for IF with cerebellum.
Boster Scientific Support
Answered: 2015-08-19
Question
I have a question about product A11407, anti-CNTN5/Contactin 5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Zhao
Verified customer
Asked: 2014-05-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11407 anti-CNTN5/Contactin 5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-19
