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Product Info Summary
| SKU: | A15678 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TYW3 Antibody
SKU/Catalog Number
A15678
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TYW3 Antibody (Catalog # A15678). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
TYW3 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TYW3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15678)
Host
Rabbit
Contents
TYW3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TYW3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TYW3. The immunogen is located within amino acids 160 - 210 of TYW3.
Cross-reactivity
TYW3 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW3 are known to exist; this antibody will detect both isoforms.
Reactive Species
A15678 is reactive to TYW3 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.8 kDa
Background of TYW3
TYW3 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15678 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TYW3 antibody can be used for detection of TYW3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TYW3 in A549 cell lysate with TYW3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Specific Publications For Anti-TYW3 Antibody (A15678)
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