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Product Info Summary
| SKU: | A03017 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-A-kinase anchor protein 13 AKAP13 Antibody
SKU/Catalog Number
A03017
Size
100ul
Form
Liquid
Description
Boster Bio Anti-A-kinase anchor protein 13 AKAP13 Antibody (Catalog# A03017). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A-kinase anchor protein 13 AKAP13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03017)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AKAP13. Immunogen sequence location: 721-770
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03017 is reactive to AKAP13 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
307.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03017 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat and A549 cells, using AKAP13 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-A-kinase anchor protein 13 AKAP13 Antibody (A03017)
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Customer Q&As
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3 Customer Q&As for Anti-A-kinase anchor protein 13 AKAP13 Antibody
Question
We are currently using anti-AKAP13 antibody A03017 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-AKAP13 antibody (A03017) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
Question
Would anti-AKAP13 antibody A03017 work on horse IHC with hippocampus?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Our lab technicians have not tested anti-AKAP13 antibody A03017 on horse. You can run a BLAST between horse and the immunogen sequence of anti-AKAP13 antibody A03017 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse hippocampus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-15
Question
Will A03017 anti-AKAP13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Roberts
Verified customer
Asked: 2015-01-22
Answer
You can see on the product datasheet, A03017 anti-AKAP13 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-01-22
