Anti-Cofilin 2/CFL2 Antibody

Cofilin 2 antibody

Boster Bio Anti-Cofilin 2/CFL2 Antibody catalog # RP1107. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: RP1107
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Cofilin 2/CFL2 Antibody

View all Cofilin 2 Antibodies

SKU/Catalog Number

RP1107

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cofilin 2/CFL2 Antibody catalog # RP1107. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cofilin 2/CFL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1107)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1107 is reactive to CFL2 in Human, Mouse, Rat

Applications

RP1107 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

18.737kDa

Background of Cofilin 2

Cofilin 2 (muscle), also known as CFL2, is a protein which in humans is encoded by the CFL2 gene. It is mapped to 14q12. This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. And this protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CFL2 (Source: Uniprot.org, NCBI)

Gene Name

CFL2

Full Name

Cofilin-2

Weight

18.737kDa

Superfamily

actin-binding proteins ADF family

Alternative Names

cofilin 2 (muscle); Cofilin, muscle isoform; cofilin-2; NEM7 CFL2 NEM7 cofilin 2 cofilin-2|cofilin 2 (muscle)|nemaline myopathy type 7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CFL2, check out the CFL2 Infographic

CFL2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CFL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Cofilin 2/CFL2 Antibody

Question

Can you help my question with product RP1107, anti-Cofilin 2/CFL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1107 anti-Cofilin 2/CFL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-01

Question

We are currently using anti-Cofilin 2/CFL2 antibody RP1107 for rat tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

The anti-Cofilin 2/CFL2 antibody (RP1107) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-06

Question

Will anti-Cofilin 2/CFL2 antibody RP1107 work for ICC with placenta skeletal muscle?

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

According to the expression profile of placenta skeletal muscle, CFL2 is highly expressed in placenta skeletal muscle. So, it is likely that anti-Cofilin 2/CFL2 antibody RP1107 will work for ICC with placenta skeletal muscle.

Boster Scientific Support

Answered: 2019-04-24

Order DetailsPrice
RP1107

100μg

$370
RP1107-10ug

10μg sample (liquid)

$99
RP1107-Biotin

100 μg Biotin conjugated

$570
RP1107-Cy3

100 μg Cy3 conjugated

$570
RP1107-Dylight488

100 μg Dylight488 conjugated

$570
RP1107-Dylight550

100 μg Dylight550 conjugated

$570
RP1107-Dylight594

100 μg Dylight594 conjugated

$570
RP1107-FITC

100 μg FITC conjugated

$570
RP1107-HRP

100 μg HRP conjugated

$570
RP1107-APC

100 μg APC conjugated

$670
RP1107-PE

100 μg PE conjugated

$670
RP1107-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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RP1107
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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