Anti-COL17A1/Collagen Xvii Antibody

Product Name

Anti-COL17A1/Collagen Xvii Antibody

View all Collagen XVII Antibodies

SKU/Catalog Number

A03031

Size

100μl

Form

Liquid

Description

Boster Bio Anti-COL17A1/Collagen Xvii Antibody catalog # A03031. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COL17A1/Collagen Xvii Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03031)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide from AA range: 481-530.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03031 is reactive to COL17A1 in Human

Applications

A03031 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

150.419kDa

Background of Collagen XVII

Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:5000-10000

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.

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Western Blot (WB) analysis of 3T3, HepG2 cells using antibody diluted at 1:500.

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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.

Gene/Protein Information For COL17A1 (Source: Uniprot.org, NCBI)

Alternative Names

180 kDa bullous pemphigoid antigen 2; BA16H23.2; BP180; BP180alpha 1 type XVII collagen; BPA-2; BPAG2; BPAG2bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); bullous pemphigoid antigen 2 (180kD); Bullous pemphigoid antigen 2; COL17A1; Collagen 17; collagen alpha-1(XVII) chain; Collagen XVII; collagen XVII, alpha-1 polypeptide; collagen, type XVII, alpha 1; Collagen-17; ERED; FLJ60881; KIAA0204; LAD-1 COL17A1 BA16H23.2, BP180, BPA-2, BPAG2, ERED, LAD-1 collagen type XVII alpha 1 chain collagen alpha-1(XVII) chain|180 kDa bullous pemphigoid antigen 2|alpha 1 type XVII collagen|bA16H23.2 (collagen, type XVII, alpha 1 (BP180))|bullous pemphigoid antigen 2 (180kD)|collagen XVII, alpha-1 polypeptide|collagen, type XVII, alpha 1|type XVII collagen alpha-1

For more info on COL17A1, check out the COL17A1 Infographic

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In this infographic, you will see the following information for COL17A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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