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- Table of Contents
COL1A2 antibody (A00624-1) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunofluorescence analysis of rat-lung tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Anti-Collagen alpha-2(I) chain COL1A2 Antibody
COL1A2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
This antibody reacts with human ROC1 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 is detected. Most cell lines expressing ROC1 can be used as a positive control. Researchers should determine optimal titers for other applications.
Product Information
Product Name
Anti-Collagen alpha-2(I) chain COL1A2 Antibody
View all COL1A2 Antibodies
View all COL1A2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen I. AA range:1-50
Cross-Reactivity
No cross reactivity with other proteins.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
129.3 kDa
Cite This Product
Anti-Collagen alpha-2(I) chain COL1A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00624-1)
Publications
Application:
Species:
Collagen-based hydrogels induce stem cell chondrogenesis and hyaline cartilage regeneration: An in vivo study
Published: 2024-07-11
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 7.70
Species: Mouse
PMID: 39002909
Exposure to micron-grade silica particles triggers pulmonary fibrosis through cell-to-cell delivery of exosomal miR-107
Published: 2024-03-22
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB,IF
Impact Factor: 7.70
Species: Mouse
PMID: 38522707
Bosutinib Laden Three-Dimensional Printed Zein Scaffolds Promote Osteogenesis
Published: 2024-03-13
Journal: ACS Applied Polymer Materials
Application: IHC
Impact Factor: 4.40
Species: Rat
Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin
Published: 2024-02-22
Journal: PLATELETS
Application: IF
Impact Factor: 2.50
Species: Human
PMID: 38385327
Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy
Published: 2023-03-31
Journal: Cell Death Discovery
Application: WB
Impact Factor: 7.00
Species: Mouse
PMID: 37002200
Transcriptomic responses of porcine cumulus cells to heat exposure during oocytes in vitro maturation
Published: 2020-12-17
Journal: MOLECULAR REPRODUCTION AND DEVELOPMENT
Application: IF
Impact Factor: 2.82
Species: Pig
PMID: 33331096
Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma
Published: 2019-04-16
Journal: Journal of Cosmetic Dermatology
Application: IF
Impact Factor: 1.31
Species: Human
PMID: 30990574
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Product Promise
We promise Anti-Collagen alpha-2(I) chain COL1A2 Antibody performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00624-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026