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Product Info Summary
| SKU: | A03869 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Collagen alpha-2(V) chain COL5A2 Antibody
SKU/Catalog Number
A03869
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Collagen alpha-2 (V) chain COL5A2 Antibody catalog # A03869. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen alpha-2(V) chain COL5A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03869)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human COL5A2. AA range:1-50
Reactive Species
A03869 is reactive to COL5A2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
144.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03869 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HepG2 cells using COL5A2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Specific Publications For Anti-Collagen alpha-2(V) chain COL5A2 Antibody (A03869)
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Customer Q&As
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6 Customer Q&As for Anti-Collagen alpha-2(V) chain COL5A2 Antibody
Question
I am looking for to test anti-COL5A2 antibody A03869 on human bone for research purposes, then I may be interested in using anti-COL5A2 antibody A03869 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-COL5A2 antibody A03869, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
Is a blocking peptide available for product anti-COL5A2 antibody (A03869)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-COL5A2 antibody (A03869). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-COL5A2 antibody A03869 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The anti-COL5A2 antibody (A03869) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
Question
I see that the anti-COL5A2 antibody A03869 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-02
Question
Can you help my question with product A03869, anti-COL5A2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Johnson
Verified customer
Asked: 2017-12-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03869 anti-COL5A2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-01
Question
Would anti-COL5A2 antibody A03869 work for WB with bone?
G. Bhatt
Verified customer
Asked: 2014-05-08
Answer
According to the expression profile of bone, COL5A2 is highly expressed in bone. So, it is likely that anti-COL5A2 antibody A03869 will work for WB with bone.
Boster Scientific Support
Answered: 2014-05-08
