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Product Info Summary
| SKU: | A00517-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Collagen Type II/COL2A1 Antibody Picoband®
SKU/Catalog Number
A00517-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen Type II/COL2A1 Antibody Picoband® catalog # A00517-2. Tested in WB,IHC,ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Collagen Type II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00517-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241). Human COL2A1 shares 92% and 96% amino acid (aa) sequence identity with mouse and rat COL2A1, respectively.
Reactive Species
A00517-2 is reactive to COL2A1 in Human, Mouse, Rat
Observed Molecular Weight
150-180 kDa
Calculated molecular weight
141.8 kDa
Background of COL2A1
Collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), also known as COL2A1, is a human gene that provides instructions for the production of the pro-alpha1(II) chain of type II collagen. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00517-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat bone tissue, mouse bone tissue
IHC: mouse knee cartilage tissue, rat knee cartilage tissue
Validation Images & Assay Conditions
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Western blot analysis of Collagen Type II/COL2A1 using anti-Collagen Type II/COL2A1 antibody (A00517-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat bone tissue lysates(100°C boiled for 5 minutes),
Lane 2: rat bone tissue lysates(37°C incubated for 10 minutes),
Lane 3: mouse bone tissue lysates(100°C boiled for 5 minutes).
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen Type II/COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen Type II/COL2A1 at approximately 150-180 kDa. The expected band size for Collagen Type II/COL2A1 is at 142 kDa.
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IHC analysis of Collagen Type II/COL2A1 using anti-Collagen Type II/COL2A1 antibody (A00517-2).
Collagen Type II/COL2A1 was detected in a paraffin-embedded section of mouse knee cartilage tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen Type II/COL2A1 Antibody (A00517-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of Collagen Type II/COL2A1 using anti-Collagen Type II/COL2A1 antibody (A00517-2).
Collagen Type II/COL2A1 was detected in a paraffin-embedded section of rat knee cartilage tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen Type II/COL2A1 Antibody (A00517-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Collagen Type II/COL2A1 Antibody Picoband® (A00517-2)
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