Anti-COLLAGEN Type V COL5A1 Antibody

Product Name

Anti-COLLAGEN Type V COL5A1 Antibody

View all Collagen V alpha 2 Antibodies

SKU/Catalog Number

A02283

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-COLLAGEN Type V COL5A1 Antibody (Catalog # A02283). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.

Storage & Handling

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-COLLAGEN Type V COL5A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02283)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Collagen Type V from human and bovine placenta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02283 is reactive to COL5A2 in Bovine, Human

Applications

A02283 is guaranteed for Dot blot, IHC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

144.91kDa

Background of Collagen V alpha 2

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:50,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:1,000 - 1:10,000

Validation Images & Assay Conditions

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Anti collagen V antibody (600-401-107 Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.0LifeSpan Biosciences, Seattle, WA

Gene/Protein Information For COL5A2 (Source: Uniprot.org, NCBI)

Alternative Names

AB collagen; collagen alpha-2(V) chain; collagen, fetal membrane, A polypeptide; collagen, type V, alpha 2; MGC105115; type V preprocollagen alpha 2 chain COL5A2 EDSC, EDSCL2 collagen type V alpha 2 chain collagen alpha-2(V) chain|AB collagen|collagen, fetal membrane, A polypeptide|type V preprocollagen alpha 2 chain

For more info on COL5A2, check out the COL5A2 Infographic

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In this infographic, you will see the following information for COL5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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