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COL10A1 antibody (M01026) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Western blot analysis of COL10A1 using anti-COL10A1 antibody (M01026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL10A1 antigen affinity purified monoclonal antibody (Catalog # M01026) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL10A1 at approximately 66 kDa. The expected band size for COL10A1 is at 66 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL10A1 antigen affinity purified monoclonal antibody (Catalog # M01026) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL10A1 at approximately 66 kDa. The expected band size for COL10A1 is at 66 kDa.
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Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody
COL10A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
COL10A1 antibody was positively detected by WB in:
mouse liver tissue, rat liver tissue, human fetal skin
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody
View all COL10A1 Antibodies
View all COL10A1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Collagen X
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
66 kDa
Calculated Molecular Weight
66.2 kDa
Cite This Product
Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01026)
Protocols
Western Blot protocol for COL10A1 antibody
Publications
Application:
Species:
CDs-PEI/siIhh Delivery System for the Treatment of Osteoarthritis
Published: 2026-05-26
Journal: ACS Applied Bio Materials
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 42187059
Rotating magnetic field downregulating type XI collagen to suppress triple-negative breast cancer metastasis by inactivating the ITGB1/FAK/YAP signaling pathway
Published: 2025-12-13
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Human
PMID: 41391796
Collagen-based hydrogels induce hyaline cartilage regeneration by immunomodulation and homeostasis maintenance
Published: 2024-07-25
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 9.40
Species: Rabbit
PMID: 39067644
TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
Published: 2024-03-16
Journal: ARTHRITIS RESEARCH & THERAPY
Impact Factor: 4.40
PMID: 38493104
A Photoannealed Granular Hydrogel Facilitating Hyaline Cartilage Regeneration via Improving Chondrogenic Phenotype
Published: 2022-09-02
Journal: ACS Applied Materials & Interfaces
Application: WB
Impact Factor: 10.38
Species: Rat
PMID: 36052731
Review
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1 Review For Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody
Anti-COL10A1 Antibody (M01026) yielded specific, clean staining at the epiphyseal line of mouse knee joint sections by IHC, consistent with its physiological role in endochondral ossification
| SKU | M01026 |
|---|---|
| Application | Immunohistochemistry |
| Sample | mouse knee joint tissue |
| Sample Processing Description | Four-week-old mice were fixed and decalcified in the knee joint, then embedded in paraffin and sectioned longitudinally. |
| Other Reagents | Goat serum, Two-step histochemistry kit |
| Primary Antibody | Collagen X COL10A1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Two-step histochemistry kit |
| Secondary Incubation | Incubate at 37°C for 45 minutes |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. IL1β is a typical pro-inflammatory factor. The experimental results showed that the target band was clear, the total IL1β protein expression was upregulated after modeling, and the expression level decreased after drug treatment, which was in line with expectations. |
Jin Sun, Tangdu Hospital, Xi’an, China
Verified customer
Submitted 2026-08-26
Product Promise
We promise Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026