Click image to see more details
Product Info Summary
| SKU: | A13263 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-Collagen XXIII alpha1 COL23A1 Antibody
SKU/Catalog Number
A13263
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen XXIII alpha1 COL23A1 Antibody (Catalog# A13263). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen XXIII alpha1 COL23A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13263)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Collagen XXIII alpha1. Immunogen sequence location: 461-510
Reactive Species
A13263 is reactive to COL23A1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
51.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13263 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using Collagen XXIII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Collagen XXIII alpha1 COL23A1 Antibody (A13263)
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Customer Q&As
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6 Customer Q&As for Anti-Collagen XXIII alpha1 COL23A1 Antibody
Question
Will anti-Collagen XXIII alpha1 antibody A13263 work on zebrafish IF with brain?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
Our lab technicians have not validated anti-Collagen XXIII alpha1 antibody A13263 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Collagen XXIII alpha1 antibody A13263 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-28
Question
Is there a BSA free version of anti-Collagen XXIII alpha1 antibody A13263 available?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen XXIII alpha1 antibody A13263 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-04
Question
Is this A13263 anti-Collagen XXIII alpha1 antibody reactive to the isotypes of COL23A1?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
The immunogen of A13263 anti-Collagen XXIII alpha1 antibody is A peptide derived from human Collagen XXIII alpha1. Immunogen sequence location: 461-510. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-17
Question
My question regarding product A13263, anti-Collagen XXIII alpha1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13263 anti-Collagen XXIII alpha1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-09
Question
We are currently using anti-Collagen XXIII alpha1 antibody A13263 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
The anti-Collagen XXIII alpha1 antibody (A13263) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-21
Question
Would A13263 anti-Collagen XXIII alpha1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Parker
Verified customer
Asked: 2015-04-29
Answer
It shows on the product datasheet, A13263 anti-Collagen XXIII alpha1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-29
