Anti-Complement C7 Antibody Picoband™

Product Name

Anti-Complement C7 Antibody Picoband™

View all Complement C7 Antibodies

SKU/Catalog Number

A00844-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Complement C7 Antibody Picoband™ catalog # A00844-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Complement C7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00844-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Complement C7 recombinant protein (Position: R233-D453).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00844-1 is reactive to C7 in Human, Mouse, Rat

Applications

A00844-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

93 kDa

Calculated molecular weight

93.518kDa

Background of Complement C7

This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Complement C7 using anti-Complement C7 antibody (A00844-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human 293T cell lysates,
Lane 2: human HepG2 cell lysates,
Lane 3: human A549 cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse small intestine tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Complement C7 antigen affinity purified polyclonal antibody (Catalog # A00844-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Complement C7 at approximately 93KD. The expected band size for Complement C7 is at 93KD.

Gene/Protein Information For C7 (Source: Uniprot.org, NCBI)

Alternative Names

complement component 7; complement component C7 C7 complement C7 complement component C7|complement component 7

For more info on C7, check out the C7 Infographic

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In this infographic, you will see the following information for C7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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