Anti-COPE Antibody Picoband™

Boster Bio Anti-COPE Antibody Picoband™ catalog # A04544. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04544
Size: 100ug/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-COPE Antibody Picoband™

See all COPE products

SKU/Catalog Number

A04544

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-COPE Antibody Picoband™ catalog # A04544. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COPE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04544)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A04544 is reactive to COPE in Human, Mouse, Rat

Applications

A04544 is guaranteed for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For COPE (Source: Uniprot.Org, NCBI)

Uniprot ID

O14579

Gene ID

11316

Gene Name

COPE

Full Name

Coatomer subunit epsilon

Weight

34.482kDa

Superfamily

COPE family

Alternative Names

coatomer epsilon subunit; coatomer protein complex, subunit epsilon; coatomer subunit epsilon; epsilon coat protein; Epsilon-coat protein; epsilon-COP; FLJ13241 COPE epsilon-COP COPI coat complex subunit epsilon coatomer subunit epsilon|coatomer epsilon subunit|coatomer protein complex subunit epsilon|epsilon coat protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COPE, check out the COPE Infographic

COPE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COPE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04544

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12 Customer Q&As for Anti-COPE Antibody Picoband™

Question

Is a blocking peptide available for product anti-COPE antibody (A04544)?

M. Anderson

Verified customer

Asked: 2020-04-27

Answer

We do provide the blocking peptide for product anti-COPE antibody (A04544). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-27

Question

Would anti-COPE antibody A04544 work for IHC-P with embryonic carcinoma?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

According to the expression profile of embryonic carcinoma, COPE is highly expressed in embryonic carcinoma. So, it is likely that anti-COPE antibody A04544 will work for IHC-P with embryonic carcinoma.

Boster Scientific Support

Answered: 2020-04-03

Question

Is this A04544 anti-COPE antibody reactive to the isotypes of COPE?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The immunogen of A04544 anti-COPE antibody is E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-31

Question

Do you have a BSA free version of anti-COPE antibody A04544 available?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-COPE antibody A04544 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-15

Question

I was wanting to use your anti-COPE antibody for IHC-P for human embryonic carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryonic carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

It shows on the product datasheet, A04544 anti-COPE antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-29

Question

I see that the anti-COPE antibody A04544 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-27

Question

I am looking for to test anti-COPE antibody A04544 on human embryonic carcinoma for research purposes, then I may be interested in using anti-COPE antibody A04544 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

The products we sell, including anti-COPE antibody A04544, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-26

Question

My question regarding product A04544, anti-COPE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04544 anti-COPE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-06

Question

Does anti-COPE antibody A04544 work on primate WB with brain?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

Our lab technicians have not validated anti-COPE antibody A04544 on primate. You can run a BLAST between primate and the immunogen sequence of anti-COPE antibody A04544 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-10

Question

Would A04544 anti-COPE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

You can see on the product datasheet, A04544 anti-COPE antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-18

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic carcinoma using anti-COPE antibody A04544. Let me know if you need anything else.

N. Jha

Verified customer

Asked: 2017-10-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-11

Question

See attached the WB image, lot number and protocol we used for embryonic carcinoma using anti-COPE antibody A04544. Please let me know if you require anything else.

H. Banerjee

Verified customer

Asked: 2013-09-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-03

Conjugation

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A04544
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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