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Product Info Summary
| SKU: | M04635-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248
SKU/Catalog Number
M04635-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Catalog # M04635-1). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04635-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM248
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Histone macroH2A1.
Cross-reactivity
This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.
Reactive Species
M04635-1 is reactive to H2afy in Human, Vertebrates
Calculated molecular weight
39.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04635-1 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Recommend Dilution
WB: 0.5 ug/mL – 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts from K562 cells, using RM248 at 1 ug/mL
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ICC result
Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (M04635-1)
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7 Customer Q&As for Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248
Question
Is this M04635-1 anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 reactive to the isotypes of H2AFY?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The immunogen of M04635-1 anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 is A peptide corresponding to the C-terminus of human Histone macroH2A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 M04635-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
Can you help my question with product M04635-1, anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Bhatt
Verified customer
Asked: 2019-11-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04635-1 anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
Is a blocking peptide available for product anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 (M04635-1)?
H. Yang
Verified customer
Asked: 2018-03-22
Answer
We do provide the blocking peptide for product anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 (M04635-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-03-22
Question
We are currently using anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 M04635-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
The anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 (M04635-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-20
Question
I was wanting to use your anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 for ELISA for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
It shows on the product datasheet, M04635-1 anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 has been tested for ELISA, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-03
Question
I see that the anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal antibody, Clone#RM248 M04635-1 works with ELISA, what is the protocol used to produce the result images on the product page?
H. Johnson
Verified customer
Asked: 2015-12-01
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-12-01
